Record Detail

Exosomes are nanovesicles derived from tumor
and normal cells that are detectable in human biological fluids,
such as plasma, and cell culture supernatants. The function of
exosome secretion from Bnormal^ cells is unclear. Although
numerous studies have investigated exosomes derived from
hematopoietic cells, little is known regarding exosomes
fromT cells, even though these cells play significant roles in
innate and acquired immunity. A CCK-8 assay was used to
examine the ability of exosomes to inhibit TE13 cell prolifer-
ation. In vitro invasion and wound healing assays were con-
ducted to explore the effects of exosomes on TE13 cell migra-
tion and invasion. A Western blottinganalys is was performed
to investigate the effects of exosomes on the expression of the
EMT-related moleculesβ-catenin, NF-κB and snail. This
study aimed to investigate the effects of exosomes from irra-
diated T cells on the human esophageal squamous cell carci-
noma (ESCC) cell line TE13 and revealed that exosomes in-
hibit the proliferation but promote the metastasis of TE13 cells
in a dose-and time-dependent manner. Furthermore, exosomes
significantly increased the expression of β-catenin, NF-κB
and snail in TE13 cells. The results of this study suggest an
important role for T cell-derived exosomes in the progression
of esophageal carcinoma: T cell-derived exosomes promote
esophageal cancer metastasis, likely by promoting the EMT