Record Detail

Short tandem repeat (STR) analysis of casework samples with low DNA content include those
resulting from the transfer of epithelial cells from the skin to an object (e.g., cells on a water bottle, or brim of a
cap), blood spatter stains, and small bone and tissue fragments. Low DNA content (LDC) samples are important in a
wide range of settings, including disaster response teams to assist in victim identification and family reunification,
military operations to identify friend or foe, criminal forensics to identify suspects and exonerate the innocent, and
medical examiner and coroner offices to identify missing persons. Processing LDC samples requires experienced
laboratory personnel, isolated workstations, and sophisticated equipment, requires transport time, and involves
complex procedures. We present a rapid DNA analysis system designed specifically to generate STR profiles from
LDC samples in field-forward settings by non-technical operators. By performing STR in the field, close to the site of
collection, rapid DNA analysis has the potential to increase throughput and to provide actionable information in
real time.