Record Detail

This study aimed to explore the effects of RACK1
gene silencing on the apoptosis and proliferation of hepatocel-
lular carcinoma (HCC) MHCC97-H cells. After transfecting
MHCC97-H cells with siRNA, RACK1 gene silencing model
was established. The cells were divided into blank group,
siRNA group and empty plasmid group, respectively. The
mRNA and protein expressions of RACK1, cyclin D1 and
BAX were determined by qRT-PCR and Western blotting.
CCK-8 assay, flow cytometry and FITC-Annexin V/PI stain-
ing were used to determine cell viability, cell cycle and cell
apoptosis, respectively. The results of qRT-PCR and Western
blotting suggested that when compared with the blank group
and the empty plasmid group, the mRNA and protein expres-
sions of RACK1 and Cyclin D1 decreased significantly while
the mRNA and protein BAX expressions increased substan-
tially in the siRNA group (all P < 0.05). The results of CCK-8
assay revealed that the siRNA group exhibited significantly
lower cell viability when compared with the blank group and
the empty plasmid group (both P < 0.05); and the cell viability
in the siRNA group decreased gradually with the increase of
time. The results of flow cytometry and FITC-Annexin V/PI
staining indicated that when compared with the blank group