Record Detail
Advanced Search
Text
Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT™ reagent
- Share to:
The INLIGHT™strategy for N-linked glycan derivatization has been shown to overcome many of the challenges associated with
glycan analysis. The hydrazide tag reacts efficiently with the glycans, increasing their non-polar surface area, allowing for
reversed-phase separations and increased ionization efficiency. We have taken the INLIGHT™ strategy and adopted it for use
with O-linked glycans. A central composite design was utilized to find optimized tagging conditions (45% acetic acid, 0.1 μg/μL
tag concentration, 37 C, 1.75 h). Derivatization at optimized conditions was much quicker than any hydrazide derivatization
strategy used previously. Human immunoglobulin A (IgA) and bovine submaxillary mucin (BSM) were then deglycosylated
through hydrazinolysis and the removed glycans were tagged under optimum conditions. XIC of tagged glycans and MS2 data
show successful hydrazide tagging of O-linked glycans for the first time.
File Attachment
Availability
| EB00000002957K | Available |
Detail Information
| Series Title |
-
|
|---|---|
| Call Number |
-
|
| Publisher | : ., |
| Collation |
-
|
| Language | |
| ISBN/ISSN |
-
|
| Classification |
NONE
|
| Content Type |
E-Jurnal
|
| Media Type |
-
|
|---|---|
| Carrier Type |
-
|
| Edition |
-
|
| Subject(s) | |
| Specific Detail Info |
-
|
| Statement of Responsibility |
-
|
Other version/related
No other version available
OPAC
RECORD DETAIL
Back To Previous
Hari Lahir Pancasila: Ajang Mengenalkan Keragaman Suku Bangsa Budaya Indonesia
Universitas Jember menggelar Upacara Bendera Peringatan Hari Lahir Pancasila 1 Juni 2024. Upacara kali ini memiliki konsep yang berbeda dari tahun sebelumnya. Jika pada tahun sebelumnya hanya jajaran pimpinan yang menggunakan pakaian adat, namun tahun ini Mahasiwa, Dosen ...
GELORA: Literasi Lingkungan, Mewarnai Masa Depan
Kegiatan Studi Literasi Eksternal hasil kolaborasi UPA Perpustakaan Universitas Jember kembali digelar pada tanggal 21-22 Juni 2024. Studi Literasi Eksternal kali ini menggunakan istilah yang berbeda yaitu GELORA (Gerakan Literasi dan Donasi Buku). Kegiatan Literasi yang berbentuk ...
BENGKEL SASTRA: MENGASAH BAKAT KEPENULISAN PUISI SANTRI JEMBER
Selasa (28/5), Santri se-Kabupaten Jember melakukan Library Tour di UPA Perpustakaan Universitas Jember. Kegiatan ini termasuk dalam serangkaian acara Bengkel Sastra yang dilaksanakan oleh Balai Bahasa Provinsi Jawa Timur. Kegiatan Bengkel Sastra dengan Tema “Penulisan Puisi Bagi Santri ...