Record Detail
Advanced Search![No image available for this title](./images/default/image.png)
Text
Changes in contractile and metabolic parameters of skeletal muscle as rats age from 3 to 12 months
Laboratory rats are considered mature at 3 months despite that musculoskeletal growth is still occurring. Changes in muscle physiological and biochemical characteristics during development from 3 months, however, are not well understood. Whole muscles and single skinned fibres from fast-twitch extensor digitorum longus (EDL) and predominantly slow-twitch soleus (SOL) muscles were examined from male Sprague–Dawley rats (3, 6, 9, 12 months). Ca2+ sensitivity of contractile apparatus decreased with age in both fast- (~ 0.04 pCa units) and slow-twitch (~ 0.07 pCa units) muscle fibres, and specific force increased (by ~50% and ~25%, respectively). Myosin heavy chain composition of EDL and SOL muscles altered to a small extent with age (decrease in MHCIIa proportion after 3 months). Glycogen content increased with age (~80% in EDL and 25% in SOL) and GLUT4 protein density decreased (~ 35 and 20%, respectively), whereas the glycogen-related enzymes were little changed. GAPDH protein content was relatively constant in both muscle types, but COXIV protein decreased~40% in SOL muscle. Calsequestrin (CSQ) and SERCA densities remained relatively constant with age, whereas there was a progressive ~ 2–3 fold increase in CSQ-like proteins, though their role and importance remain unclear. There was also ~40% decrease in the density of the Na+, K+-ATPase (NKA) α1 subunit in EDL and the α2 subunit in SOL. These findings emphasise there are substantial changes in skeletal muscle function and the density of key proteins during early to mid-adulthood in rats, which need to be considered in the design and interpretation of experiments.
File Attachment
Availability
EB00000003578K | Available | ||
EB00000003579K | Available |
Detail Information
Series Title |
-
|
---|---|
Call Number |
-
|
Publisher | : ., |
Collation |
-
|
Language | |
ISBN/ISSN |
-
|
Classification |
NONE
|
Content Type |
E-Jurnal
|
Media Type |
-
|
---|---|
Carrier Type |
-
|
Edition |
-
|
Subject(s) | |
Specific Detail Info |
-
|
Statement of Responsibility |
Hongyang Xu1, Graham D. Lamb2, Robyn M. Murphy1
|
Other version/related
No other version available
OPAC
RECORD DETAIL
Back To Previous
Hari Lahir Pancasila: Ajang Mengenalkan Keragaman Suku Bangsa Budaya Indonesia
Universitas Jember menggelar Upacara Bendera Peringatan Hari Lahir Pancasila 1 Juni 2024. Upacara kali ini memiliki konsep yang berbeda dari tahun sebelumnya. Jika pada tahun sebelumnya hanya jajaran pimpinan yang menggunakan pakaian adat, namun tahun ini Mahasiwa, Dosen ...
GELORA: Literasi Lingkungan, Mewarnai Masa Depan
Kegiatan Studi Literasi Eksternal hasil kolaborasi UPA Perpustakaan Universitas Jember kembali digelar pada tanggal 21-22 Juni 2024. Studi Literasi Eksternal kali ini menggunakan istilah yang berbeda yaitu GELORA (Gerakan Literasi dan Donasi Buku). Kegiatan Literasi yang berbentuk ...
BENGKEL SASTRA: MENGASAH BAKAT KEPENULISAN PUISI SANTRI JEMBER
Selasa (28/5), Santri se-Kabupaten Jember melakukan Library Tour di UPA Perpustakaan Universitas Jember. Kegiatan ini termasuk dalam serangkaian acara Bengkel Sastra yang dilaksanakan oleh Balai Bahasa Provinsi Jawa Timur. Kegiatan Bengkel Sastra dengan Tema “Penulisan Puisi Bagi Santri ...