Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell
experimental methods and biological engineering applications. Although a variety of cell patterning methods have
been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain
phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally
perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires
no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.

• Laser-fabricated cell patterning stencil for single cell analysis