This article presents a PCB based microfluidic plat-
form for performing a dielectrophoretic capture of live micro-
organisms over inter-digitated electrodes buried under layers
of different surface roughness values. Although dielec-
trophoresis has been extensively studied earlier over silicon
and polymer surfaces with printed electrodes the issue of sur-
face roughness particularly in case of buried electrodes has
been seldom investigated. We have addressed this issue
through a layer of spin coated PDMS (of various surface
roughness) that is used to cover the printed electrodes over a
printed circuit board. The roughness in the PDMS layer is
generally defined by the roughness of the FR4 base which
houses the printed electrodes as well as other structures.
Possibilities arising out of COMSOL simulations have been
well validated experimentally in this work.

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• Impact of surface roughness on Dielectrophoretically assisted concentration of microorganisms over PCB based platforms