Cell migration assays for different chemical envi-
ronments are important for both scientists and clinicians
searching for new therapeutics. In this study, we developed a
multi-well-based microfluidic chip that has multiple units for
different conditions. In each unit, cells can be patterned and
then released to observe their migration. Automatic image
analysis and model-based data processing were developed to
describe the integrated cell migration assay precisely and
quickly. As a demonstration, the migration behaviors of two
types of cells in eight chemical conditions were studied. The
results showed that supplementation with transforming
growth factor-β(TGF-β) significantly promoted the migration
of MCF-7 and MCF-10 A cells compared to several growth
factors, such as Epidermal Growth Factor(EGF) and basic
fibroblast growth factor(bFGF), as well as a control sample.
Cells can migrate particularly fast with two or more mixed
supplementary factors, such as TGF-β + bFGF + EGF, which

• A parallel and quantitative cell migration assay using a novel multi-well-based device