Ectomycorrhizal (ECM) and ectendomycorrhizal
fungal species associated with Pinus montezumae were re-
corded in 8 year-old trees established in microcosms and com-
pared with those associated with 2 year-old trees, in order to
determine their persistence over the long-term. Mycorrhizal
root tips were morphologically and anatomically character-
ized and sequenced. The extension of extramatrical mycelium
of ECM fungi with long exploration strategies was evaluated.
In total, 11 mycorrhizal species were registered. Seven mycor-
rhizal species were detected on both 2 and 8 year-old pines:
Atheliaceae sp., Rhizopogon aff. fallax, R. aff. occidentalis,
Suillus pseudobrevipes, Tuber separans, Wilcoxina mikolae
and Wilcoxina rehmii. One species, Thelephora terrestris,
was exclusively associated with two year–old seedlings, while
Cenococcum geophilum, Pezizaceae sp. and Pyrenomataceae
sp. were exclusively found on 8 year-old trees. Atheliaceae sp.
was the ECM fungal species that presented the most abundant
mycelium. Finally, we report one new fungal species of
Pezizaceae occurring as a symbiont of P. montezumae.

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• Persistence of ecto- and ectendomycorrhizal fungi associated with Pinus montezumae in experimental microcosms