In this paper, we have fabricated 3D carbon nano-
fiber microelectrode arrays (MEAs) with highly reproducible
and rich chemical surface areas for fast scan cyclic voltamm-
etry (FSCV). Carbon nanofibers are created from negative
photoresist by a new process called dual O 2 plasma-assisted
pyrolysis. The proposed approach significantly improves film
adhesion and increases surface reactivity. We showcase our
sensor’s compatibility with FSCV analysis by demonstrating
highly sensitive and stable FSCV dopamine measurements on
a prototype 4-channel array. We envision with proper surface
fuctionalization the 3D carbon nanofiber MEA enable sensi-
tive and reliable detection of multiple neurotransmitters
simultaneously.

• 3D carbon nanofiber microelectrode arrays fabricated by plasma-assisted pyrolysis to enhance sensitivity and stability of real-time dopamine detection