In this work, the effect of ascorbic and gibberellic acids and pH of medium on embryogenesis and
regeneration in anther culture of spring triticale was studied. The study of androgenesis was conducted on two
model samples YaTKh-327-11 and YaTKh-18-11. The research revealed that the addition of the antioxidant
ascorbic acid at a concentration of 2–8 mg/L into the culture medium to induce embryogenesis enhanced the
formation of androgen structures and regeneration of green plants. The addition of gibberellic acid into the
culture medium to induce embryogenesis gave negative effects. The average yield of green plants in the anther
culture of spring triticale was 2 pcs/100 anthers. According to the research, 277 green plants were received and
spontaneous doubling of chromosomes was recorded in 26.7%.

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• Influence of Different Concentrations of Ascorbic and Gibberellic Acids and pH of Medium on Embryogenesis and Regeneration in Anther Culture of Spring Triticale