Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals
and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC–MS is often used for
the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review
provides insight into the LC–MS methods used for the determination of co-occurring mycotoxins with special emphasis on
multiple-analyte applications. The first part of the review is focused on targeted LC–MS approaches using cleanup methods such
as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy,
cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted
determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the
fate of mycotoxins in plants