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UPA Perpustakaan Universitas Jember

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

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An integrated microsystem device with matched
interdigitated microelectrode chip was fabricated for enrich-
ment and detection of Escherichia coli O157:H7. The
microsystem has integrated with positive dielectrophoresis
(pDEP) enrichment and in situ impedance detection, whose
total volume is only 3.0 × 10 −3 m 3 , and could provide im-
pedance testing voltages of 0 ~ 10 V, detection frequencies
of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude
of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which
fully meets the demands of pDEP enrichment and imped-
ance detection for bacteria. The microfluidic chip with inter-
digitated m icroelectrodes w as manufac tu red by
microfabrication methods. The interdigital microelectrode ar-
ray has sufficient contact area with a bacterial suspension to
improve enrichment efficiency and detection sensitivity.
Bacteria in the interdigital microelectrode area of the
microfluidic chip were firstly captured and enriched by
pDEP. Then, in situ impedance detection of the enriched
bacteria was realized by switching test conditions. Using

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