We report the fabrication of a label free nano
biosensor platform comprising single nanofiber that is
derived out of multi-walled carbon nanotubes (MWC-
NTs) embedded SU-8 photoresist, for the detection of
three important human cardiac biomarkers viz., myoglobin
(Myo), cardiac Troponin I (cTn I) and Creatine Kinase-
MB (CK-MB). These composite nanofibers were synthe-
sized using electrospinning process. Single nanofibers were
aligned between pairs of electrodes in-situ during the elec-
trospinning process. The target proteins were detected using
chemiresistive detection methodology. Each biomarker was
detected using a specific, single, aligned nanofiber, func-
tionalized with its corresponding monoclonal antibody.
Chemiresistive detection involves measuring the change in
conductance of the functionalized nanofibers upon the bind-
ing of the targeted antigen. The minimum detection limits
of Myo, CK-MB and cTn I were experimentally found
out to be as low as 6, 20 and 50 fg/ml respectively. No
response was observed when the nanofibers were exposed
to a non-specific protein, demonstrating excellent speci-
ficity to the targeted detection. These MWCNTs embedded
SU-8 nanofibers based nanobiosensor platform shows great
promise in the detection of cardiac markers and other pro-
teins as they have fast response time, high sensitivity and
good specificity.