Microfluidic perfusion systems (MPS) are well
suited to perform multiparametric measurements with small
amounts of tissue to function as an Organ on Chip device
(OOC). Such microphysiolgical characterization is particular-
ly valuable in research on the stimulus-secretion-coupling of
pancreatic islets. Pancreatic islets are fully functional compe-
tent mini-organs, which serve as fuel sensors and transduce
metabolic activity into rates of hormone secretion. To enable
the simultaneous measurement of fluorescence and oxygen
consumption we designed a microfluidic perfusion system
from borosilicate glass by 3D femtosecond laser ablation.
Retention of islets was accomplished by a plain well design.
The characteristics of flow and shear force in the
microchannels and wells were simulated and compared with
the measured exchange of the perfusion media. Distribution of
latex beads, MIN6 cell pseudo islets and isolated mouse islets
in the MPS was characterized in dependence of flow rate and
well depth. Overall, the observations suggested that a

• A 3D microfluidic perfusion system made from glass for multiparametric analysis of stimulus-secretion coupling in pancreatic islets